PENGARUH LIMBAH CAIR NANAS (LCN) TERHADAP PERTUMBUHAN DAN KANDUNGAN LEMAK CHLORELLA SP

Leli Triana1 dan Agus Sutanto2 (2016) Pengaruh Limbah Cair Nanas (lcn) Terhadap Pertumbuhan Dan Kandungan Lemak Chlorella Sp

Jurnal Lentera Pendidikan Pusat Penelitian Lppm Um Metro Vol. 1. No. 2, Desember 2016


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ABSTRAK

Abstract

The purpose of this study was to determine (1) Analyze the influence of the variation

media of pineapple liquid waste (PLW) to increase the growth of Chlorella sp. (2)

Analyze the influece the variation of media to increase the fat of Chlorella sp. (3) To

determine Media Concentration of pineapple liquid waste to optimal level for the

growth of Chlorella sp. (4) Determine the optimum concentration of pineapple liquid

waste for the highest fat content in Microalgae (Chlorella sp.) This study uses design

experimental method, design of the design used is Variety Complete Randomized

Design (CRD) with one control (PLW 0%), and 5 treatments PLW 20%,40%,60%,80%

and 100% which each four times repeated. 5 ml Chlorella sp. The early density 8 x104

cell/ml inoculated in 100 ml PLW. For each culture media were cultivated for 10

days. The calculation Chlorella sp. Conducted which calculated the amount of cell/day

as long as cultivation. Meanwhile the calculation of fat content conducted at the end of

cultivation. Anlyzed used Multy-Variant.Based on study result and explanation were

got conclusion (1) Significant influence of variation media pineapple liquid waste

toward the growth and fat content of Chlorella sp. (2) Cell density of Chlorella was

highest in pineapple liquid waste media 100% (3) The highest fat content was found in

concentratin media 60% pineapple liquid waste.

Keywords: Pineapple Liquid Waste, Media,Chlrella sp. Growth, Fat Content.

Amini,S. Dan R.Susilowati. (2010).

Produksi Biodisel dari Mikroalga

Jurnal Lentera Pendidikan Pusat Penelitian LPPM UM METRO Vol. 1. No. 2, Desember 2016 178

Botrococcus braunii. Squalen Vol

: 23-32

Becker EW, Baddiley SJ, Carey NH,

Higgins IJ, Potter WG. (1995).

Microalgae :Biotechnology and

microbiology. New York :

Cambridge University Press.

Chalid, S.Y., S. Amini, dan S.D.

Lestari. (2010). Kultivasi

Chlorella sp. padaMedia

Tumbuh yang Diperkaya

dengan Pupuk Anorganik dan

Soil Extract. FST, UIN

Syarif Hidayatullah Press,

Jakarta : 398 – 304.

Cohen, Z. (1999). Chemicals from

Microalgae. Israel: Ben Gurion

University of The Negev

Hills, Nakamura. (1978). Food from

Sunlight World Hunger

Research Project, University of

The Trees Press.

Kimball , JW. (1991). Biology . Jilid 1

edisi 5 Erlangga. Jakarta.

Kawaroe M, Prartono T, Sunuddin A,

Sari DW, Augustine D. (2010).

Mikroalga: Potensi dan

Pemanfaatannya untuk Produksi

Bio Bahan Bakar. Bogor: PT

Penerbit IPB Press.

Lestari, S.D. (2010). Kultivasi dan

Ekstraksi Minyak Nabati dari

Mikroalga Laut Jenis

Nannochloropsis sp dan

Spirulina plantesis Dengan

Metode Pengepresan dan Bligh

Dyer.Jakarta. Universitas Islam

Negeri Syarif Hidayatullah.

Poejiadi, Anna.(1994-1996).

Kumpulan

Makalah Tentang Literasi Sains

Dan

Teknologi. Fakultas Pendidikan

MIPA. IKIP Bandung.

Sutanto, Agus. (2009). Isolasi Bakteri

Indigenous Limbah Cair Nanas

Berpotensi Bioremediasi, Jurnal

Bioedukasi, Volume VII (1), 56-

66

Sutanto, Agus. (2011). Degradasi

Bahan Organik Limbah Cair

Nanas Oleh Bakteri Indigen.

Metro: UM El-Hayah. Vol. 1,No.

4, hlm 151-156.

Vonshak, A. S. Boussiba; A.

Abeliovich & A. Richmond.

(2004). Production of Sprirulina

platensis biomass: Maintenance

of monoalgal culture outdoors.

Biotech. and Bioengineering. 25

(2): 341-349.


REFERENSI

Amini,S. Dan R.Susilowati. (2010).

Produksi Biodisel dari Mikroalga

Jurnal Lentera Pendidikan Pusat Penelitian LPPM UM METRO Vol. 1. No. 2, Desember 2016 178

Botrococcus braunii. Squalen Vol

: 23-32

Becker EW, Baddiley SJ, Carey NH,

Higgins IJ, Potter WG. (1995).

Microalgae :Biotechnology and

microbiology. New York :

Cambridge University Press.

Chalid, S.Y., S. Amini, dan S.D.

Lestari. (2010). Kultivasi

Chlorella sp. padaMedia

Tumbuh yang Diperkaya

dengan Pupuk Anorganik dan

Soil Extract. FST, UIN

Syarif Hidayatullah Press,

Jakarta : 398 – 304.

Cohen, Z. (1999). Chemicals from

Microalgae. Israel: Ben Gurion

University of The Negev

Hills, Nakamura. (1978). Food from

Sunlight World Hunger

Research Project, University of

The Trees Press.

Kimball , JW. (1991). Biology . Jilid 1

edisi 5 Erlangga. Jakarta.

Kawaroe M, Prartono T, Sunuddin A,

Sari DW, Augustine D. (2010).

Mikroalga: Potensi dan

Pemanfaatannya untuk Produksi

Bio Bahan Bakar. Bogor: PT

Penerbit IPB Press.

Lestari, S.D. (2010). Kultivasi dan

Ekstraksi Minyak Nabati dari

Mikroalga Laut Jenis

Nannochloropsis sp dan

Spirulina plantesis Dengan

Metode Pengepresan dan Bligh

Dyer.Jakarta. Universitas Islam

Negeri Syarif Hidayatullah.

Poejiadi, Anna.(1994-1996).

Kumpulan

Makalah Tentang Literasi Sains

Dan

Teknologi. Fakultas Pendidikan

MIPA. IKIP Bandung.

Sutanto, Agus. (2009). Isolasi Bakteri

Indigenous Limbah Cair Nanas

Berpotensi Bioremediasi, Jurnal

Bioedukasi, Volume VII (1), 56-

66

Sutanto, Agus. (2011). Degradasi

Bahan Organik Limbah Cair

Nanas Oleh Bakteri Indigen.

Metro: UM El-Hayah. Vol. 1,No.

4, hlm 151-156.

Vonshak, A. S. Boussiba; A.

Abeliovich & A. Richmond.

(2004). Production of Sprirulina

platensis biomass: Maintenance

of monoalgal culture outdoors.

Biotech. and Bioengineering. 25

(2): 341-349.